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E-ISSN: 1308-6308 ISSN: 1304-9054
Evaluation of Platelet Functions in Pediatric Patients with Chronic Renal Failure
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Original Article
VOLUME: 17 ISSUE: 2
P: 220-231
August 2019

Evaluation of Platelet Functions in Pediatric Patients with Chronic Renal Failure

J Curr Pediatr 2019;17(2):220-231
DOI: 10.4274/jcp.2019.0020
Funda Tayfun Küpesiz 1
Canan Vergin 2
Mustafa Bak 3
1. Sağlık Bilimleri Üniversitesi Antalya Eğitim Araştırma Hastanesi Çocuk Hematoloji Bölümü
2. Sağlık Bilimleri Üniversitesi Dr. Behçet Uz Çocuk Hastalıkları ve Cerrahisi Eğitim ve Araştırma Hastanesi Çocuk Hematoloji Bölümü
3. İzmir Çocuk Nefrolojisi Kliniği
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ABSTRACT

DISCUSSION and CONCLUSION:

The PFA 100 method, which is easier than the flow cytometry method for the evaluation of primary hemostasis due to platelet dysfunction in uremic patients, may prove to be an ideal method for the general evaluation of primary hemostasis in the event of bleeding in uremic patients.

RESULTS:

PFA 100 analysis showed that median closure time was significantly higher among hemodialysis patients in terms of collagen/epinephrine, collagen/ADP comparisons (p=0.000, p=0.000); however, these values returned to normal after hemodialysis (p= 0.018, p = 0.028). The in vitro bleeding time of patients undergoing peritoneal dialysis was found to be in the normal range. The in vitro bleeding time improvements were better in peritoneal dialysis compared to hemodialysis. Analysis with flow cytometry showed that; GP Ib (CD42b mAb) levels in dialysis patients were significantly higher than control group and predialysis patients (p=0.037). The fibrinogen receptor (GPIIb) level decreased significantly in hemodialysis patients,after hemodialysis (p = 0.018).

METHODS:

This study was a prospective evaluation of patients who were followed by the Nephrology Clinic of Behçet Uz Children's Diseases and Pediatric Surgery Training and Research Hospital with a diagnosis of chronic renal failure. The study group consisted of 57 patients and 31 healthy controls. Platelet surface fibrinogen (GP IIb-IIa) and von Willebrand Factor (vWF) receptors (GP Ib-IX) were measured by flow cytometry. In vitro bleeding time was measured by the platelet function analyzer method (PFA 100).

INTRODUCTION:

Patients with chronic renal failure may tend to bleed in relation to the severity and duration of uremia. Currently, effective dialysis procedures and the removal of uremic toxins that cause platelet dysfunction partially alleviate bleeding disorders. In this study, our aim was to evaluate bleeding, platelet dysfunction, and factors that were effective on platelet dysfunction in patients with chronic renal failure.